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Related Experiment Video

Updated: Jun 2, 2026

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

Two-photon mapping of neural circuits.

Volodymyr Nikolenko, Elodie Fino, Rafael Yuste

Cold Spring Harbor Protocols

|May 4, 2011

Summary

Researchers developed a new method to map neural circuits by identifying presynaptic neurons in brain slices. This technique offers single-cell resolution for functional analysis of neuronal connectivity.

Area of Science:

Neuroscience
Cellular Biology
Neurophysiology

Background:

Understanding neural circuits requires identifying neuronal interconnections in living preparations.
Current methods may lack the resolution or speed for comprehensive connectivity analysis.

Purpose of the Study:

To develop a method for detecting presynaptic neurons to a target cell in brain slices.
To enable systematic and rapid functional analysis of neuronal connectivity.

Main Methods:

Utilized two-photon uncaging of glutamate in brain slices.
Developed a technique to identify neurons presynaptic to a specific cell.

Main Results:

Achieved single-cell resolution in mapping neuronal connections.

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Related Experiment Videos

Last Updated: Jun 2, 2026

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

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Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals

Published on: January 19, 2024

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Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

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Demonstrated the ability to systematically test neuronal populations.

Enabled online functional analysis of neural connectivity.

Conclusions:

The developed method allows for precise identification of presynaptic partners.
This technique facilitates rapid and comprehensive mapping of neural circuits.
Offers a powerful tool for understanding neural network organization and function.