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Updated: Jun 2, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
10:42

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

Published on: December 31, 2017

[Buccal epithelium reactivity in children]

A V Kurkin, D Kh Rybalkina

    Morfologiia (Saint Petersburg, Russia)
    |May 5, 2011
    PubMed

    Insights

    Buccal smears reveal distinct cellular changes in adolescents and children with chronic tonsillitis or chicken pox. Adolescent cells mature faster, while illness increases leukocytes and inflammation markers in buccal mucosa.

    Area of Science:

    • Oral cytology
    • Pediatric pathology
    • Cellular differentiation

    Context:

    • Buccal mucosa analysis provides insights into physiological and pathological changes.
    • Understanding age-related cellular variations is crucial for pediatric health assessments.
    • Cytological examination aids in diagnosing inflammatory and infectious conditions.

    Purpose:

    • To analyze buccal smears from healthy children and those with chronic tonsillitis and chicken pox.
    • To identify age-specific cytological peculiarities in the buccal mucosa.
    • To characterize cytological changes associated with chronic tonsillitis and chicken pox.

    Summary:

    • Adolescents exhibit a higher proportion of mature buccal epithelial cells (stages 5 and 6) with reduced cell and nuclear area.
    • Children with chronic tonsillitis and chicken pox show increased leukocytes, altered epithelial cell proportions, and elevated inflammation-destruction indexes.
    • Pappenheim's staining method was employed for cytological analysis of buccal smears.

    Impact:

    • Establishes normative cytological data for adolescent buccal mucosa.
    • Highlights potential cytological biomarkers for pediatric inflammatory and infectious diseases.
    • Informs diagnostic approaches for common childhood illnesses affecting oral mucosa.

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