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Linkage of a RAPD marker with powdery mildew resistance
1Nuasheen University of Malakand, Department of Botany, Khyber Pakhtunkhwa, Pakistan. mnshaalpk@yahoo.com
Genetika
|May 5, 2011
Summary
Powdery mildew resistance in pea plants is inherited in a monogenic recessive pattern. A DNA marker, OPB18, was identified and linked to the resistance gene for use in marker-assisted selection (MAS) breeding programs.
Area of Science:
- Plant genetics
- Molecular breeding
- Plant pathology
Background:
- Powdery mildew is a significant disease affecting pea (Pisum sativum) crops.
- Developing resistant cultivars is crucial for sustainable agriculture.
- Marker-assisted selection (MAS) can accelerate breeding for disease resistance.
Purpose of the Study:
- To investigate the inheritance pattern of powdery mildew resistance in Pisum sativum.
- To identify and tag the resistance gene with a DNA marker.
- To facilitate marker-assisted selection (MAS) for breeding resistant pea varieties.
Main Methods:
- Artificial inoculation of pea germplasm with Erysiphe pisi to identify resistant and susceptible genotypes.
- Crossing resistant (Fallon) and susceptible (11760-3ER) genotypes to generate F1 and F2 generations.
- Segregation analysis of F2 plants and Random Amplified Polymorphic DNA (RAPD) marker analysis.
Main Results:
- Powdery mildew resistance in Pisum sativum follows monogenic recessive inheritance.
- The susceptible allele demonstrated dominance over the resistant allele in F1 generation.
- A novel RAPD marker, OPB18, showed linkage to the powdery mildew resistance gene (er-1) at a distance of 11.2 cM with 83% probability and a LOD score of 4.13.
Conclusions:
- The er-1 gene confers monogenic recessive resistance to powdery mildew in pea.
- The identified RAPD marker OPB18 is a valuable tool for MAS in pea breeding programs.
- This study provides a foundation for marker-assisted development of powdery mildew-resistant pea cultivars.
