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Fluorescence Lifetime Macro Imager for Biomedical Applications
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Photoswitchable nanoprobes offer unlimited brightness in frequency-domain imaging

Alexander D Q Li1, Chuanlang Zhan, Dehong Hu

  • 1Department of Chemistry and Center for Materials Research, Washington State University, Pullman, Washington 99164, USA. dequan@wsu.edu

Journal of the American Chemical Society
|May 5, 2011
PubMed

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.