Related Experiment Video

Updated: Jun 2, 2026

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

Inhibition effect of siRNA-downregulated UHRF1 on breast cancer growth

Feng Yan1, Xu-Yan Tan, Yao Geng

  • 1Department of Clinical Laboratory, Jiangsu Institute of Cancer Prevention and Cure, Nanjing, People's Republic of China. yanfeng2007@sohu.com

Cancer Biotherapy & Radiopharmaceuticals
|May 5, 2011
PubMed

Insights

Downregulating the UHRF1 gene using a lentiviral shRNA system significantly inhibits breast cancer cell proliferation and colony formation. This RNA interference approach shows promise for effective breast cancer therapy.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • The UHRF1 gene is implicated in cell proliferation and multidrug resistance in breast cancer.
  • Targeting UHRF1 may offer a novel therapeutic strategy for breast cancer treatment.

Purpose of the Study:

  • To investigate the effect of UHRF1 downregulation on breast cancer cell growth and proliferation.
  • To evaluate the potential of an shRNA lentiviral system for targeting UHRF1 in breast cancer therapy.

Main Methods:

  • Utilized a short-hairpin RNA (shRNA) lentiviral system to downregulate UHRF1 expression in MDA-MB-231 and MCF-7 breast cancer cell lines.
  • Assessed cell proliferation using methylthiazole tetrazolium (MTT), bromodeoxyuridine (BrdU) incorporation, and colony formation assays.

Main Results:

  • UHRF1 downregulation led to a significant decrease in the proliferative potential of breast cancer cells.
  • Inhibition of UHRF1 expression markedly reduced the colony formation capacity of both cell lines.
  • The shRNA lentiviral system effectively reduced UHRF1 levels in the targeted cells.

Conclusions:

  • RNA interference targeting UHRF1 is a viable strategy to inhibit breast cancer cell proliferation.
  • The developed shRNA lentiviral system demonstrates potential as an effective therapeutic approach for breast cancer treatment.

More Related Videos

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...