Related Experiment Video
Updated: Jun 2, 2026
12:55
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
High affinity, developability and functional size: the holy grail of combinatorial antibody library generation
Dirk Ponsel1, Julia Neugebauer, Kathrin Ladetzki-Baehs
1MorphoSys AG, Lena-Christ Strasse 48, 82152 Martinsried, Germany.
Molecules (Basel, Switzerland)
|May 5, 2011
Summary
Antibody library design has evolved from focusing on affinity to encompass developability, production efficiency, and reduced immunogenicity for improved therapeutic antibody generation using phage display technology.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- Phage display technology is crucial for generating human antibodies.
- The quality of antibody libraries is paramount for in vitro selection methods.
- Library design has evolved significantly since the technology's inception.
Purpose of the Study:
- To review the evolution of antibody library design in phage display.
- To highlight the shift in focus from affinity to developability and safety.
- To discuss recent re-evaluations of library design for improved therapeutic outcomes.
Main Methods:
- Review of historical development and advancements in antibody library generation.
- Analysis of different library types (naïve, synthetic) and their characteristics.
- Examination of selection strategies and their impact on library quality.
Main Results:
- Early libraries prioritized affinity through size and diversity.
- Increased focus on developability, cost of goods, and biophysical properties.
- Recent efforts concentrate on reducing immunogenicity and enhancing biophysical behavior.
Conclusions:
- Antibody library design is a dynamic field adapting to therapeutic needs.
- Modern library development balances affinity with crucial developability and safety factors.
- Re-evaluation of library design is essential for creating superior therapeutic antibodies.
More Related Videos
Related Concept Videos
01:41Affinity and Avidity
Overview
01:31Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
01:28Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:26Antibody Actions
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
01:03Affinity Chromatography
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...