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Updated: Jun 2, 2026

Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
Oncogenesis in HIV-infection
A Albini1, M Aluigi, R Benelli
1UNIV MILAN,DERMATOL CLIN 1,I-20157 MILAN,ITALY. KAROLINSKA HOSP & INST,IMMUNOPATHOL LAB,S-17176 STOCKHOLM,SWEDEN. MAX PLANCK INST BIOCHEM,ABT VIRUSFORSCH,D-82152 MARTINSRIED,GERMANY. UNIV PADUA,IST ONCOL,I-35128 PADUA,ITALY. HOP ST LOUIS,F-75010 PARIS,FRANCE. UNIV ERLANGEN NURNBERG,INST KLIN & MOLEK VIROL,D-91054 ERLANGEN,GERMANY. L SACCO HOSP VIALBA,DEPT PATHOL,I-20157 MILAN,ITALY. IST NAZL RIC CANC,LAB ONCOL MED,I-16132 GENOA,ITALY. KAROLINSKA HOSP & INST,NEUROGENET LAB,S-17176 STOCKHOLM,SWEDEN. UNIV VIENNA,SCH MED,DEPT DERMATOL,DIV IMMUNOL ALLERGY & INFECT DIS,A-1090 VIENNA,AUSTRIA.
Abstract:
The presence of human Kaposi's sarcoma associated herpesvirus-like sequences (KSHV) was examined in different epidemiological variants of Kaposi's sarcoma (KS) and in KS-derived cell cultures by polymerase chain reaction (PCR). KSHV DNA was present in all tumor biopsies of AIDS-associated KS (59 biopsies), endemic KS (26 biopsies; 21 African endemic KS, 5 Greek endemic KS), sporadic/classical KS (28 biopsies) and post-transplant/iatrogenic KS (6 of 7 biopsies). On the contrary, these sequences were only detected rarely in non-involved skin of KS patients (3 positive specimens of 12), in the peripheral blood mononuclear cells of HIV-infected patients (3 positive specimens of 54) and in lymphoma-biopsies (3 positive specimens of 47). Cell cultures derived from KS skin lesions were positive for KSHV DNA only in the first two passages. However, two longer-term positive cultures from a biopsy of a patient affected with sporadic KS and a biopsy of a patient affected with epidemic KS was identified. A strong association of KSHV with KS tissue was observed in all the different epidemiological variants of KS. Long-term positive KS-derived cell cultures will be an important tool to study the herpesvirus-like agent and to investigate its functional role in the initiation and progression of KS.
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