Related Experiment Video
Updated: Jun 2, 2026
09:13
Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
Oncogenesis in HIV-infection.
A Albini1, M Aluigi, R Benelli
1UNIV MILAN,DERMATOL CLIN 1,I-20157 MILAN,ITALY. KAROLINSKA HOSP & INST,IMMUNOPATHOL LAB,S-17176 STOCKHOLM,SWEDEN. MAX PLANCK INST BIOCHEM,ABT VIRUSFORSCH,D-82152 MARTINSRIED,GERMANY. UNIV PADUA,IST ONCOL,I-35128 PADUA,ITALY. HOP ST LOUIS,F-75010 PARIS,FRANCE. UNIV ERLANGEN NURNBERG,INST KLIN & MOLEK VIROL,D-91054 ERLANGEN,GERMANY. L SACCO HOSP VIALBA,DEPT PATHOL,I-20157 MILAN,ITALY. IST NAZL RIC CANC,LAB ONCOL MED,I-16132 GENOA,ITALY. KAROLINSKA HOSP & INST,NEUROGENET LAB,S-17176 STOCKHOLM,SWEDEN. UNIV VIENNA,SCH MED,DEPT DERMATOL,DIV IMMUNOL ALLERGY & INFECT DIS,A-1090 VIENNA,AUSTRIA.
International Journal of Oncology
|May 5, 2011
Summary
Human Kaposi
Area of Science:
- Virology
- Oncology
- Dermatology
Background:
- Kaposi's sarcoma (KS) is a complex malignancy with diverse epidemiological presentations.
- The role of human Kaposi's sarcoma associated herpesvirus-like virus (KSHV) in KS pathogenesis requires further investigation across different KS variants.
Purpose of the Study:
- To investigate the presence and association of KSHV in various epidemiological subtypes of Kaposi's sarcoma.
- To evaluate KSHV detection in KS-derived cell cultures for potential research applications.
Main Methods:
- Polymerase chain reaction (PCR) was employed to detect KSHV DNA.
- Analysis included tumor biopsies from AIDS-associated, endemic, sporadic, and post-transplant KS.
- KSHV detection was also assessed in non-involved skin, peripheral blood mononuclear cells, lymphoma biopsies, and KS-derived cell cultures.
Main Results:
- KSHV DNA was detected in all examined KS tumor biopsies across all epidemiological variants.
- KSHV sequences were rarely found in non-KS tissues and peripheral blood.
- While initially transient, long-term KSHV-positive KS-derived cell cultures were established from sporadic and epidemic KS biopsies.
Conclusions:
- A strong association between KSHV and Kaposi's sarcoma tissue is confirmed across all epidemiological variants.
- Long-term KSHV-positive KS cell cultures represent a valuable resource for studying KSHV's role in KS initiation and progression.
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