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Autocrine motility factor and its receptor expressions in human oral squamous cell carcinoma (SCC) cells

Y Niinaka1, S Oida, A Ishisaki

  • 1KARMANOS CANC INST,TUMOR PROGRESS & METASTASIS PROGRAM,DETROIT,MI 48201. WAYNE STATE UNIV,DEPT RADIAT ONCOL,DETROIT,MI 48201. WAYNE STATE UNIV,DEPT PATHOL,DETROIT,MI 48201. UNIV TOKYO,FAC DENT,DEPT BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN. UNIV TOKYO,FAC DENT,DEPT ORAL & MAXILLOFACIAL SURG,BUNKYO KU,TOKYO 113,JAPAN. TORIDE KYODO GEN HOSP,DEPT ORAL SURG,TORIDE,IBARAKI 302,JAPAN. GUNMA UNIV,SCH MED,DEPT ORTHOPAED SURG,MAEBASHI,GUMMA 371,JAPAN.

International Journal of Oncology
|May 5, 2011
PubMed

Insights

Oral squamous-cell carcinoma (SCC) cells produce autocrine motility factor (AMF) and its receptor (AMFR). AMF and AMFR expression correlates with SCC cell invasiveness and metastatic potential.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • Autocrine motility factor (AMF) is a cytokine secreted by tumors that promotes cancer cell motility.
  • AMF interacts with its receptor, AMFR (gp78), initiating a signal transduction pathway.
  • The role of AMF and AMFR in oral squamous-cell carcinoma (SCC) metastasis requires further elucidation.

Purpose of the Study:

  • To investigate the production and expression of AMF and AMFR in human oral squamous-cell carcinoma (SCC) cells.
  • To determine the relationship between AMF/AMFR expression and the metastatic potential of SCC cells.

Main Methods:

  • Culturing of metastatic LMF4 human oral squamous-cell carcinoma (SCC) cells.
  • Dose- and time-dependent induction of morphological changes and chemotaxis by secreted AMF.
  • Analysis of AMFR mRNA expression in SCC cell variants with varying metastatic abilities.

Main Results:

  • SCC cells were shown to secrete AMF.
  • Secreted AMF induced dose- and time-dependent morphological changes and chemotaxis in the producing SCC cells.
  • AMFR mRNA expression was significantly associated with the metastatic capacity of SCC cell variants.

Conclusions:

  • Human oral squamous-cell carcinoma (SCC) cells produce both AMF and its receptor, AMFR.
  • The expression of AMF and AMFR in SCC cells is directly related to their invasiveness and metastatic potential.
  • These findings highlight AMF/AMFR signaling as a potential therapeutic target for oral cancer metastasis.

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