[Genetic polymorphisms of 21 non-CODIS STR loci].
Wei-bo Shao1, Su-hua Zhang, Li Li
1Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. swbcy0131@sina.com
Fa Yi Xue Za Zhi
|May 6, 2011
Summary
Genetic analysis of 21 non-CODIS short tandem repeat (STR) loci in the Han population reveals high polymorphism. These STR loci demonstrate significant forensic application value for personal identification and paternity testing.
Area of Science:
- Forensic genetics
- Population genetics
- Molecular biology
Background:
- Short tandem repeat (STR) loci are crucial markers in forensic science.
- Non-CODIS STR loci offer additional discriminatory power beyond standard databases.
- Understanding population-specific genetic variation is essential for accurate forensic analysis.
Purpose of the Study:
- To characterize the genetic polymorphisms of 21 non-CODIS STR loci in the Han population from eastern China.
- To evaluate the forensic applicability of these STR loci for individual identification and relationship testing.
Main Methods:
- DNA samples from 225 unrelated Han individuals were analyzed.
- Twenty-one non-CODIS STR loci were amplified using the AGCU 21+1 STR kit.
- Genotyping was performed using a 3130 Genetic Analyzer, with data analyzed by GeneMapper ID v3.2, PowerStats v12.xls, and Cervus 2.0 software.
Main Results:
- All 21 non-CODIS STR loci exhibited distributions consistent with Hardy-Weinberg equilibrium.
- High levels of heterozygosity (0.596-0.804) and polymorphic information content (0.522-0.807) were observed.
- Exceptional discrimination power (0.764-0.948) and cumulative probabilities of exclusion (duo-testing: 0.999707, trios-testing: 0.9999994) were achieved.
Conclusions:
- The 21 non-CODIS STR loci are highly polymorphic and suitable for forensic applications.
- These loci are effective for personal identification and paternity testing in trio cases.
- They serve as valuable supplements for challenging diad paternity testing scenarios.
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