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Three-Dimensional (3D) Tumor Spheroid Invasion Assay
Published on: May 1, 2015
Tenascin in human neoplasia.
P Shrestha1, M Kusakabe, M Mori
1ASAHI UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG 1,GIFU,JAPAN. INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,TSUKUBA,IBARAKI 305,JAPAN.
International Journal of Oncology
|May 6, 2011
Summary
Tenascin (TN) is upregulated in most human cancers, potentially influencing tumor growth and metastasis. This study found enhanced TN expression in 94% of neoplastic lesions, regardless of origin.
Area of Science:
- Extracellular matrix biology
- Cancer research
- Immunohistochemistry
Background:
- Tenascin (TN) is an extracellular matrix protein primarily involved in embryonic development.
- Its role and expression in adult tissues and neoplastic lesions are not well understood.
- TN expression is known to be restricted in normal adult tissues but altered in disease states.
Purpose of the Study:
- To investigate the expression patterns of Tenascin (TN) in various human neoplastic lesions.
- To determine the correlation between TN expression and clinicopathological features of tumors.
- To assess the potential role of TN in tumor progression and metastasis.
Main Methods:
- Immunohistochemistry was used to evaluate TN immunoreactivity in 1050 human neoplasia tissue specimens.
- Seven different monoclonal antibodies against TN were employed in a streptavidin-biotin immunoperoxidase method.
- Fetal and normal adult tissues were used as controls for comparison.
Main Results:
- Enhanced TN expression was observed in hyperplastic and dysplastic epithelial lesions, correlating with the degree of abnormality.
- Widespread stromal immunoreactivity for TN was found in squamous cell carcinoma and adenocarcinoma, affecting tumor characteristics.
- Non-epithelial tumors also exhibited enhanced TN expression; overall, 94% of neoplastic cases showed increased TN immunoreactivity.
Conclusions:
- Enhanced in vivo expression of Tenascin (TN) is a common feature across a wide spectrum of neoplastic lesions.
- Altered TN expression may significantly impact tumor growth, differentiation, vascularity, cellular adhesion, invasion, and metastasis.
- Further research combining in vitro and in vivo findings is warranted to elucidate TN's precise molecular mechanisms in cancer.
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