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Sequential expression of myc-, ras-, oncogene products and EGF receptor during DMBA-induced tongue carcinogenesis

S Shintani, T Matsumura, R Alcalde

    International Journal of Oncology
    |May 6, 2011
    PubMed

    Insights

    This study investigated oral cancer development using a hamster model. Key proteins like c-myc and epidermal growth factor receptor (EGFr) showed altered expression during tumor formation, suggesting their role in oral carcinogenesis.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Carcinogenesis Research

    Background:

    • The precise mechanisms driving oral cancer, particularly epidermoid carcinomas, remain incompletely understood.
    • Genetic alternations are implicated in the development of oral squamous cell carcinomas.

    Purpose of the Study:

    • To investigate the expression patterns of c-myc, c-Ha-ras proteins, and epidermal growth factor receptor (EGFr) during 9,10-dimethyl 1,2-benzanthracene (DMBA)-induced oral carcinogenesis.
    • To elucidate the roles of these proteins in the stages of epithelial dysplasia and tumor progression in a hamster tongue model.

    Main Methods:

    • Utilized a hamster tongue model for DMBA-induced oral carcinogenesis.
    • Examined the protein expression levels of c-myc, c-Ha-ras, and EGFr through immunohistochemistry during different stages of carcinogenesis.

    Main Results:

    • c-myc protein expression increased with epithelial dysplasia and throughout tumorigenesis.
    • c-Ha-ras protein expression decreased from normal epithelium to squamous cell carcinomas, being nearly absent in tumors.
    • Epidermal growth factor receptor (EGFr) overexpression was detected early (1-4 weeks) after DMBA treatment and increased during carcinogenesis.

    Conclusions:

    • c-myc and c-Ha-ras protein expression changes are significant in oral malignant transformation.
    • EGFr overexpression is correlated with very early stages of oral tumor development in this in vivo model.

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