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Updated: Jun 2, 2026

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

Expression and sequence analysis of the SDI1/WAF1/CIP1/p21 tumor suppressor gene in prostate cancer cell lines

C Papandreou1, T Bogenrieder, H Scher

  • 1MEM SLOAN KETTERING CANC CTR,GENITOURINARY ONCOL RES LAB,NEW YORK,NY 10021. MEM SLOAN KETTERING CANC CTR,LAB MAMMALIAN CELL TRANSFORMAT,NEW YORK,NY 10021. MEM SLOAN KETTERING CANC CTR,DEPT MED,DIV SOLID TUMOR ONCOL,GENITOURINARY ONCOL SERV,NEW YORK,NY 10021. CORNELL UNIV,MED CTR,NEW YORK,NY 10021.

International Journal of Oncology
|May 6, 2011
PubMed

Insights

Prostate cancer cell lines express p21 mRNA and protein, but lack mutations in the SDI1/WAF1/CIP1/p21 gene. This suggests p21 gene defects are uncommon in prostate cancers.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • The SDI1/WAF1/CIP1 gene encodes p21, a protein that inhibits cyclin-dependent kinases to arrest cell growth.
  • p21 is a key downstream effector in the p53 pathway and is upregulated by wild-type p53.
  • Somatic mutations in the p21 gene occur in 17% of primary prostate cancers.

Purpose of the Study:

  • To investigate the expression and mutation status of the p21 gene in prostate cancer cell lines.
  • To determine if mutations in the p21 gene are present in cultured prostate cancer cells.

Main Methods:

  • Analysis of p21 mRNA and protein expression in four prostate cancer cell lines.
  • Mutation screening of the entire coding region of the p21 gene using SSCP analysis and direct DNA sequencing.

Main Results:

  • p21 mRNA was detected in all examined prostate cancer cell lines.
  • p21 protein was found in LNCaP, DU-145, and TSU-Pr1 cells, but not in PC-3 cells.
  • No mutations were identified in the coding domains of the p21 gene across the tested cell lines.

Conclusions:

  • Mutations in the SDI1/WAF1/CIP1/p21 gene are absent in the studied prostate cancer cell lines.
  • These findings suggest that defects in the p21 gene are infrequent in prostate cancers.

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