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Three serotypes of Norwalk-like virus demonstrated by solid-phase immune electron microscopy
1Public Health Laboratory, Musgrove Park Hospital, Taunton, Somerset, England.
Journal of Medical Virology
|January 1, 1990
Summary
Solid phase immune electron microscopy (SPIEM) confirmed two Norwalk-like virus (NLV) serotypes and identified a third. SPIEM is effective for typing NLV strains and detecting specific IgM, aiding in seroconversion confirmation.
Area of Science:
- Virology
- Immunology
- Epidemiology
Background:
- Norwalk-like viruses (NLVs) are a significant cause of gastroenteritis outbreaks.
- Serological characterization is crucial for understanding NLV epidemiology and strain diversity.
Purpose of the Study:
- To investigate serological differences among NLV strains from UK outbreaks.
- To evaluate the utility of Solid Phase Immune Electron Microscopy (SPIEM) for NLV serotyping.
Main Methods:
- Solid Phase Immune Electron Microscopy (SPIEM) was employed.
- Whole convalescent sera and purified IgM were used for typing.
- Paired sera were analyzed to detect antibody rises.
Main Results:
- Two previously identified NLV serotypes were confirmed.
- A third distinct serotype was identified as the cause of recent hospital outbreaks.
- SPIEM successfully differentiated NLV serotypes and detected specific IgM responses.
- Antibody rises correlated with infecting serotype, though cross-reactivity was observed.
Conclusions:
- SPIEM is a valuable tool for distinguishing NLV serotypes and confirming seroconversion.
- Cross-reacting antibodies can occur, potentially complicating typing without further investigation.
- Understanding NLV serotypes is essential for effective outbreak investigation and control.