Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Anatomy of Chloroplasts01:08

The Anatomy of Chloroplasts

Green algae and plants, including green stems and unripe fruit, harbor specialized organelles called chloroplasts to carry out photosynthesis. They coordinate both stages of photosynthesis — the light-dependent reactions and the light-independent reactions. The light-dependent reactions use sunlight to release oxygen and produce chemical energy in the form of ATP and NADPH, and the light-independent reactions capture CO2 and use ATP and NADPH to produce sugar.
Structure of Chloroplasts
A...
Anatomy of Chloroplasts01:07

Anatomy of Chloroplasts

Green algae and plants, including green stems and unripe fruit, harbor chloroplasts—the vital organelles where photosynthesis takes place. In plants, the highest density of chloroplasts is found in the mesophyll cells of leaves.
Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Protein Transport to the Stroma01:24

Protein Transport to the Stroma

Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein Transport to the Thylakoids01:22

Protein Transport to the Thylakoids

Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oral Delivery of Bioencapsulated CTB-Pro-IGF-1 Enhances Bone Regeneration in a Pre-Clinical Rat Model of Jaw Osteoradionecrosis.

Oral diseases·2026
Same author

Ex vivo HNSCC clinical studies using saliva and antiviral or antibacterial chewing gums reveal reduction in carcinogenic microbes.

Scientific reports·2026
Same author

Engineering Marker-Free Lettuce Chloroplast Genome to Express Functional Glucagon-Like Peptide-1 Receptor Agonists Exenatide and Lixisenatide.

Plant biotechnology journal·2026
Same author

Phylogeny, chromosomal mapping and expression analyses of wheat CLAVATA pathway components suggest differential selection on receptor-like kinases, CLEs and T3 WOXes.

The Plant journal : for cell and molecular biology·2025
Same author

Advanced Dialogues: From Laboratory to Clinics: Plant Cell-Based Affordable Biologics.

Advanced genetics (Hoboken, N.J.)·2025
Same author

Expression of mannanase and glucanases in lettuce chloroplasts and functional evaluation of enzyme cocktail against Candida albicans in oral cancer patient samples.

Plant biotechnology journal·2025
Same journal

Production of Functional Trastuzumab in Phaeodactylum tricornutum: Advancing Microalgae as Next-Generation Sustainable Antibody Biofactories.

Plant biotechnology journal·2026
Same journal

A Fusion Between Common Bean Phaseolin and the N-Terminal Domain of Maize 16 kDa Gamma Zein: Clues for Designing Nutritionally Improved Seed Storage Proteins.

Plant biotechnology journal·2026
Same journal

CRISPR/Cas9-Mediated Editing of Four Ca<sup>2+</sup>-Binding Receptor-Like Cytoplasmic Kinases Improves Soybean Seed Size and Yield at Dense Planting.

Plant biotechnology journal·2026
Same journal

A Robust Framework for Maize Elite Line Genome Editing Through Enhanced HI-Edit via LbCas12a Activity Optimization.

Plant biotechnology journal·2026
Same journal

Insights Into the Origin and Local Adaptation Evolution of the Cultivated Sesame With Telomere-to-Telomere High-Quality Genome.

Plant biotechnology journal·2026
Same journal

Development of In Vitro and In Planta Strategies for Efficient Production of High-Value Steviol Glycosides.

Plant biotechnology journal·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
10:28

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Published on: October 19, 2018

Preface: chloroplast biotechnology

Henry Daniell, Keith J Edwards

    Plant Biotechnology Journal
    |May 7, 2011
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    Studying Protein Import into Chloroplasts Using Protoplasts
    06:29

    Studying Protein Import into Chloroplasts Using Protoplasts

    Published on: December 10, 2018

    A Robotic Platform for High-throughput Protoplast Isolation and Transformation
    10:12

    A Robotic Platform for High-throughput Protoplast Isolation and Transformation

    Published on: September 27, 2016

    Related Experiment Videos

    Last Updated: Jun 2, 2026

    Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
    10:28

    Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

    Published on: October 19, 2018

    Studying Protein Import into Chloroplasts Using Protoplasts
    06:29

    Studying Protein Import into Chloroplasts Using Protoplasts

    Published on: December 10, 2018

    A Robotic Platform for High-throughput Protoplast Isolation and Transformation
    10:12

    A Robotic Platform for High-throughput Protoplast Isolation and Transformation

    Published on: September 27, 2016