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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jun 2, 2026

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified &#955; DNA at the Single Molecule Level
08:56

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

One-to-one quantum dot-labeled single long DNA probes.

Shibin He1, Bi-Hai Huang, Junjun Tan

  • 1State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, PR China.

Biomaterials
|May 7, 2011
PubMed
Summary

Researchers developed quantum dot (QD)-based polymerase chain reaction (PCR) to create fluorescent DNA probes. This QD-PCR method enables single-molecule detection and imaging for advanced diagnostics and biological research.

More Related Videos

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Related Experiment Videos

Last Updated: Jun 2, 2026

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified &#955; DNA at the Single Molecule Level
08:56

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Area of Science:

  • Nanobiotechnology
  • Molecular Biology
  • Materials Science

Background:

  • Quantum dots (QDs) possess unique optical properties, making them attractive for bio-labeling.
  • Conjugating biomolecules to QDs is a key goal in nanobiotechnology for advanced applications.
  • Developing methods for precise QD-DNA conjugation is crucial for sensitive detection.

Purpose of the Study:

  • To develop a novel polymerase chain reaction (PCR) method using QDs for DNA amplification.
  • To characterize the resulting QD-DNA conjugates for their structure, fluorescence, and detection capabilities.
  • To demonstrate the utility of QD-labeled DNA probes in biological applications like gene detection.

Main Methods:

  • Developed QD-based PCR (QD-PCR) by amplifying primer oligonucleotides bound to QDs.
  • Characterized QD-PCR products using gel electrophoresis and atomic force microscopy (AFM).
  • Utilized QD-labeled DNA conjugates for fluorescence in situ hybridization (FISH) on maize chromosomes.

Main Results:

  • QD-PCR successfully synthesized QD-labeled long DNA strands with a one-to-one QD-DNA ratio.
  • The synthesized QD-DNA conjugates retained their fluorescence properties.
  • One-to-one QD-labeled DNA probes accurately detected single-copy genes on maize chromosomes via FISH.

Conclusions:

  • QD-based PCR provides a robust method for creating single QD-labeled DNA conjugates.
  • These conjugates serve as sensitive fluorescent probes for detecting single-copy DNA fragments.
  • This nanotechnology advancement holds significant potential for medical diagnostics, biological research, and nano-material fabrication.