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Updated: Jun 2, 2026

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties

Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties

Published on: January 8, 2016

Ratiometric temperature sensing with semiconducting polymer dots.

Fangmao Ye¹, Changfeng Wu, Yuhui Jin

¹Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.

Journal of the American Chemical Society

|May 10, 2011

Summary

This summary is machine-generated.

Researchers developed ultrabright semiconducting polymer dots (Pdots) for ratiometric temperature sensing. These Pdot-RhB nanoparticles offer sensitive, linear temperature measurement within cells and can also serve as fluorescent probes.

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Last Updated: Jun 2, 2026

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Published on: January 8, 2016

Thermal Measurement Techniques in Analytical Microfluidic Devices

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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

Area of Science:

Nanotechnology
Biophysics
Materials Science

Background:

Developing nanoscale sensors for precise temperature monitoring is crucial for biological research.
Existing methods often lack the sensitivity, brightness, or ratiometric capabilities needed for intracellular measurements.

Purpose of the Study:

To create ultrabright, single-nanoparticle ratiometric temperature sensors using semiconducting polymer dots (Pdots).
To evaluate the sensor's performance for measuring intracellular temperatures in live cells.

Main Methods:

Synthesizing semiconducting polymer dots (Pdots) incorporating the temperature-sensitive dye Rhodamine B (RhB).
Characterizing the Pdot-RhB nanoparticles for brightness, temperature sensitivity, and ratiometric performance.
Utilizing the Pdot-RhB nanoparticles for live-cell imaging and intracellular temperature measurements.

Main Results:

The Pdot-RhB nanoparticles exhibited excellent temperature sensitivity and high brightness due to Pdot light harvesting and energy transfer.
The sensors demonstrated ratiometric temperature sensing under single-wavelength excitation with a linear range suitable for physiological temperatures.
Intracellular temperatures were successfully measured in live cells using the Pdot-RhB nanoparticles.

Conclusions:

Ultrabright Pdot-RhB nanoparticles serve as effective nanoscale ratiometric temperature sensors.
These sensors are suitable for accurate intracellular temperature measurements and live-cell imaging.
The technology offers a promising tool for biological and biomedical applications requiring precise temperature monitoring.