Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ERGA-BGE reference genome of <i>Gambusia holbrooki</i>, a globally invasive freshwater fish.

Open research Europe·2026
Same author

Chromosome-level genomes of scleractinian corals: gene prediction and functional annotation.

Scientific data·2026
Same author

Genome evolution in parthenogenetic nematodes shaped by chromosome rearrangements and introgression.

Genome research·2026
Same author

Elevated retrocopy burden and sloth-specific expansions illuminate mammalian genome evolution.

BMC biology·2026
Same author

LepEU: A Consortium to Study the Population Genomics of Butterflies and Moths Across Europe.

Evolutionary applications·2026
Same author

Comparative Transcriptomics Reveals an Extracellular Worm Argonaute as an Ancestral Regulator of LTR Retrotransposons.

Genome biology and evolution·2026
Same journal

Hidden digenean diversity in two African protected areas: metabarcoding as a tool for parasite identification.

International journal for parasitology·2026
Same journal

Nearly Bitten, Twice Shy: Effects of prior and current parasite exposure on host mating behaviour.

International journal for parasitology·2026
Same journal

Bringing order to chaos: A new classification of avian schistosomes and their genetic lineages in the context of swimmer's itch.

International journal for parasitology·2026
Same journal

Assessing myxozoan diversity in fish: microscopy-based detection versus high-throughput amplicon sequencing.

International journal for parasitology·2026
Same journal

Spatial expression analysis and target prediction give insight into microRNA function in the model cestode Hymenolepis microstoma.

International journal for parasitology·2026
Same journal

Climatic suitability and phylogeography reveal cryptic diversity and public health risk in the Amblyomma testudinarium complex and other Asian Amblyomma.

International journal for parasitology·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
09:10

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

Published on: March 1, 2022

NEMBASE4: the nematode transcriptome resource.

Benjamin Elsworth1, James Wasmuth, Mark Blaxter

  • 1Institute of Evolutionary Biology, The University of Edinburgh, Edinburgh EH9 3JT, UK.

International Journal for Parasitology
|May 10, 2011
PubMed
Summary
This summary is machine-generated.

NEMBASE4 offers a unified database of over 60 nematode species' transcriptomes, aiding research into parasitic nematodes and their essential ecosystem roles. This resource facilitates the discovery of unique genes and potential drug targets.

More Related Videos

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
06:54

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Published on: March 9, 2022

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Related Experiment Videos

Last Updated: Jun 2, 2026

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
09:10

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

Published on: March 1, 2022

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus
06:54

Application of RNA Interference in the Pinewood Nematode, Bursaphelenchus xylophilus

Published on: March 9, 2022

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Area of Science:

  • Nematode genomics and bioinformatics.
  • Comparative transcriptomics.
  • Parasitology and ecology.

Background:

  • Nematode parasites impact human health and agriculture; free-living species are vital for ecosystems.
  • Existing expressed sequence tag (EST) datasets are phylogenetically diverse but difficult to compare.
  • A centralized, annotated resource is needed for nematode transcriptome data.

Purpose of the Study:

  • To create NEMBASE4, a single portal for functionally annotated EST-derived transcriptomes from over 60 nematode species.
  • To enable comparative analysis of nematode transcriptomes.
  • To identify unique genes and protein families for potential drug targets and understanding parasitic adaptations.

Main Methods:

  • Assembled publicly available ESTs using the PartiGene suite of tools.
  • Translated assembled transcripts to generate protein sequences.
  • Annotated sequences using comparisons with known nematode and non-nematode resources.
  • Assigned protein families through cross-comparison within NEMBASE4.

Main Results:

  • Developed NEMBASE4, an openly accessible, interactive database of annotated nematode transcriptomes.
  • Generated a protein sequence resource with functional annotations and family assignments.
  • Identified lineage-restricted genes in parasitic nematodes and potential drug targets.

Conclusions:

  • NEMBASE4 provides a valuable, unified resource for nematode research.
  • The database facilitates the study of parasitic adaptations and the identification of novel therapeutic targets.
  • Comparative transcriptomics within NEMBASE4 can reveal unique biological features of different nematode clades.