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Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Updated: Jun 2, 2026

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

Va-es-bj - an epithelioid sarcoma cell-line.

C Helson¹, M Melamed, S Braverman

¹NEW YORK BLOOD CTR,DEPT PATHOL,DIV HEMATOL ONCOL,VALHALLA,NY 10595. ST AGNES HOSP N ST,NEUROBLASTOMA RES LAB,WHITE PLAINS,NY.

International Journal of Oncology

|May 10, 2011

Summary

A new epithelioid sarcoma cell line, VA-ES-BJ, was established from patient bone marrow. This cell line exhibits rapid growth and secretes granulocyte macrophage colony-stimulating factor, aiding sarcoma research.

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Area of Science:

Oncology
Cell Biology
Pathology

Background:

Epithelioid sarcoma is a rare soft tissue sarcoma.
Metastatic epithelioid sarcoma can present with diverse symptoms, including cord compression.
Establishing new cell lines is crucial for understanding tumor biology and developing treatments.

Purpose of the Study:

To establish and characterize a novel human epithelioid sarcoma cell line.
To investigate the growth characteristics and secretory functions of the new cell line.
To provide a valuable research tool for epithelioid sarcoma studies.

Main Methods:

Derivation of a cell line from bone marrow aspirate of a patient with metastatic epithelioid sarcoma.
Morphological characterization of the cell line.
Immunohistochemical staining for epithelial and mesenchymal markers (AE-1, AE-3, vimentin, EMA).
Karyotyping to assess chromosomal abnormalities.
Assessment of growth in nude mice and secretion of granulocyte macrophage colony-stimulating factor (GM-CSF).

Main Results:

Successfully established and designated the epithelioid sarcoma cell line VA-ES-BJ.
The cell line consists of mononuclear and multinucleated giant cells.
Cells exhibited positive staining for cytokeratin markers (AE-1, AE-3), vimentin, and epithelial membrane antigen, confirming epithelial differentiation.
Chromosomal analysis revealed triploidy with marker chromosomes.
The cell line demonstrated rapid growth in nude mice and secreted functional granulocyte macrophage colony-stimulating factor (GM-CSF).

Conclusions:

The VA-ES-BJ cell line represents a valuable model for epithelioid sarcoma research.
Its ability to secrete GM-CSF suggests potential roles in tumor microenvironment modulation.
This cell line will facilitate studies on epithelioid sarcoma pathogenesis, metastasis, and therapeutic strategies.