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Updated: Jun 2, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
Pkc-dependent and pkc-independent cellular effects induced by recombinant transforming growth-factor type-Beta-1
S Strauss1, M Weber, C Schachtele
1UNIV FREIBURG,INST MED MIKROBIOL & HYG,VIROL ABT,D-79104 FREIBURG,GERMANY. INST MOLEK MED & NAT STOFFORSCH,TUMORBIOL KLIN,FREIBURG,GERMANY.
Abstract:
TGF-beta 1 purified from human platelets induces colony formation of rat fibroblasts reversibly when administered together with EGF. TGF-beta 1 has been reported by us to lead to stable transformation of rat fibroblasts, to activate latent Epstein-Barr virus, to exhibit tumor promoting activity for murine fibroblasts, and to cause the release of apoptosis-inducing signals from normal cells which are directed specifically against transformed cells. The use of recombinant TGF-beta 1 in this study showed that all the effects measured were indeed due to TGF-beta 1. In order to get information on intracellular signal pathways induced by TGF-beta 1, specific inhibitors of protein kinase C (PKC) were applied. Induction of EBV antigens by TGF-beta 1 was dependent on the function of PKC, whereas induction of apoptosis in transformed cells by TGF-beta 1-treated normal cells was independent of the action of PKC.
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