Related Experiment Video
Updated: Jun 2, 2026
Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis
Published on: October 13, 2023
Image cytometry for DNA analysis in endometrial carcinoma correlated with other prognostic parameters
M Giovagnoli1, A Lukic, R Muraro
1UNIV ROMA LA SAPIENZA,DEPT EXPTL MED,ROME,ITALY. UNIV ROMA LA SAPIENZA,DEPT OBSTET & GYNECOL 2,ROME,ITALY. UNIV G DANNUNZIO,INST HUMAN PATHOL & SOCIAL MED 1,CHIETI,ITALY.
Abstract:
We evaluated by static cytometry DNA ploidy parameters in 30 stage I-IV endometrial carcinomas and correlated these data with standard clinical-pathological features and disease-free period. We observed a direct correlation between either non-diploid DNA content and deeper myometrial invasion (p<0.02) or D.I.greater than or equal to 1.2 and M2-M3 tumors (p<0.009). The Kaplan Meier survival curves illustrate a more rapid relapse of disease associated with non-diploidy, high 5cExR, high level of proliferation and D.I.greater than or equal to 1.2. while Cox regression model gave relative hazards for disease recurrence of 4, 6, 3.7 and 2.1 for non-diploidy, D.I.greater than or equal to 1.2, high 5cExR and high level of proliferation respectively. This prospective study confirmed the prognostic value of DNA Index, and its usefulness in clinical practice especially in stage I cases, otherwise characterised by favourable standard prognostic factors, is discussed.
