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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
An efficient calcium phosphate nanoparticle-based nonviral vector for gene delivery.
Yachun Liu1, Tao Wang, Fangli He
1Key Laboratory of Protein Chemistry and Developmental Biology, Ministry of Education of China, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.
International Journal of Nanomedicine
|May 11, 2011
Summary
Protamine sulfate-coated calcium phosphate (PS-CaP) nanoparticles are smaller and more effective for DNA delivery than traditional calcium phosphate methods. These enhanced nanoparticles improve transfection efficiency and stability, offering a superior nonviral gene delivery vector.
Area of Science:
- Nanotechnology
- Biotechnology
- Gene Delivery
Background:
- Smaller nanoparticles enhance cellular DNA delivery via endocytosis, improving transfection.
- Investigating protamine sulfate-coated calcium phosphate (PS-CaP) for particle stabilization and transfection efficiency.
Purpose of the Study:
- To evaluate PS-CaP nanoparticles as a nonviral gene delivery vector.
- To compare the transfection efficiency and stability of PS-CaP with classical calcium phosphate.
Main Methods:
- Utilized pEGFP-C1 for transfection efficiency assessment.
- Employed atomic force microscopy for particle morphology and size analysis.
- Assessed cell viability and inhibition using an MTT assay.
Main Results:
- PS-CaP nanoparticles exhibited smaller sizes compared to classical calcium phosphate particles.
- PS-CaP demonstrated significantly higher transfection efficiency in 293 FT, HEK 293, and NIH 3T3 cells.
- PS-CaP showed improved stability upon storage and comparable low cell toxicity.
Conclusions:
- PS-CaP represents a superior nonviral transfection vector compared to classical calcium phosphate.
- The enhanced properties of PS-CaP are attributed to protamine sulfate coating, improving DNA delivery and nanoparticle stability.
