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Transcriptional antagonism between p53 and sv40 T-antigen

S Deb, D Brown, H Lega

    International Journal of Oncology
    |May 11, 2011
    PubMed

    Insights

    Overexpression of human TATA-binding protein (TBP) activates p53-mediated transcription. However, TBP does not resolve the transcriptional antagonism between p53 and SV40 T antigen, suggesting a different inhibitory mechanism.

    Area of Science:

    • Molecular Biology
    • Transcriptional Regulation
    • Oncogenes

    Background:

    • The TATA-binding protein (TBP) is a crucial transcription factor.
    • Wild-type p53 is a tumor suppressor protein involved in transcriptional regulation.
    • Simian virus 40 (SV40) T antigen is a viral oncoprotein that can alter cellular transcription.

    Purpose of the Study:

    • To investigate the role of TBP in p53-mediated transcriptional activation.
    • To explore the interaction between p53, SV40 T antigen, and TBP in regulating transcription.

    Main Methods:

    • In vivo transient transfection-transcription assays were employed.
    • Overexpression of human TBP was utilized.
    • Saos-2 cells were used to assess p53-dependent activation.

    Main Results:

    • Overexpression of TBP activated a synthetic promoter with p53-binding sites in the presence of wild-type p53, but not in its absence.
    • Wild-type p53 inhibited SV40 late promoter transactivation by SV40 T antigen.
    • Overexpression of TBP did not relieve the inhibition between p53 and T antigen.

    Conclusions:

    • TBP may be a limiting factor for p53-mediated activation.
    • p53 likely interacts with TBP to activate promoters containing p53-binding sites.
    • The transcriptional antagonism between p53 and SV40 T antigen does not involve competition for TBP and may result from a direct inhibitory interaction between the proteins.

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