Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Limits on Trigonometric Functions01:25

Limits on Trigonometric Functions

39
Limits on Trigonometric FunctionsThe limits of trigonometric functions play a fundamental role in calculus, particularly in defining derivatives. One of the most important results is:which is important for differentiating trigonometric functions and is widely applied in mathematical analysis and physics.Geometric IntuitionA common approach to proving this result involves analyzing a sector of a unit circle with an angle subtended at the center. Since the arc length is numerically equal to the...
39
The Number e as a Limit01:29

The Number e as a Limit

53
The number e is a fundamental constant in calculus, playing a central role in describing continuous change, particularly exponential growth. It is most naturally defined through its relationship with the natural logarithm, which is the inverse of the exponential function with base e. This relationship allows e to be characterized using basic principles of differentiation rather than as an arbitrary numerical constant.A key property of the natural logarithm function, ln x, is that its derivative...
53
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae06:29

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

6.8K
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo...
6.8K
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

11.2K
A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.
11.2K
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis06:59

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

12.4K
Gene function can be obscured in loss-of-function experiments if there is compensation by another gene. The zebrafish model provides a relatively high-throughput means to reveal such functional redundancy in living...
12.4K
Limiting Reactant02:27

Limiting Reactant

69.4K
The relative amounts of reactants and products represented in a balanced chemical equation are often referred to as stoichiometric amounts. However, in reality, the reactants are not always present in the stoichiometric amounts indicated by the balanced equation.
69.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fragmented rRNAs and lineage-specific RNA-binding proteins define myzozoan mitoribosomes.

Trends in parasitology·2026
Same author

An in vivo fitness gene of Toxoplasma, MIC11, is essential for PLP1-mediated egress from host cells.

Nature communications·2026
Same author

Structural and regulatory principles underlying apicomplexan conoid function.

Trends in parasitology·2026
Same author

A Spinster-like Transporter at the Inner Membrane Complex is critical for Toxoplasma gondii cytokinesis, motility and invasion.

PLoS pathogens·2026
Same author

Metabolic needs and capabilities among Cryptosporidium species.

Trends in parasitology·2025
Same author

RNG2 tethers the conoid to the apical polar ring in Toxoplasma gondii to enable parasite motility and invasion.

PLoS biology·2025
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Limits on Trigonometric Functions
01:25

Limits on Trigonometric Functions

Published on: January 12, 2026

39

Functional genetics in Apicomplexa: potentials and limits.

Julien Limenitakis1, Dominique Soldati-Favre

  • 1Department of Microbiology and Molecular Medicine, CMU, University of Geneva, Geneva, Switzerland. Julien.Limenitakis@unige.ch

FEBS Letters
|May 12, 2011
PubMed
Summary
This summary is machine-generated.

Apicomplexan parasites cause severe diseases. This review details recent genetic manipulation strategies for Plasmodium falciparum, Plasmodium berghei, and Toxoplasma gondii, focusing on improving gene function identification.

More Related Videos

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
06:29

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Published on: April 15, 2021

6.8K
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

11.2K

Related Experiment Videos

Last Updated: Jan 20, 2026

Limits on Trigonometric Functions
01:25

Limits on Trigonometric Functions

Published on: January 12, 2026

39
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
06:29

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Published on: April 15, 2021

6.8K
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

11.2K

Area of Science:

  • Parasitology
  • Molecular Biology
  • Genetics

Background:

  • Apicomplexans are obligate intracellular protozoan parasites responsible for significant human and animal diseases.
  • Despite available genome sequences, many genes have unassigned functions, hindering research.
  • Genetic manipulation is restricted to a few Apicomplexan species with established culture or animal models.

Purpose of the Study:

  • To compare and contrast recent strategies for the genetic manipulation of Plasmodium falciparum, Plasmodium berghei, and Toxoplasma gondii.
  • To highlight approaches leveraging the unique genomic features of these parasites.
  • To discuss advancements in studying essential genes and high-throughput methods for gene function identification.

Main Methods:

  • Review of recent literature on genetic manipulation techniques in Apicomplexans.
  • Focus on strategies adapted to the specific genomic characteristics of Plasmodium and Toxoplasma species.
  • Discussion of methods for improving transfection efficiency and studying gene function.

Main Results:

  • Identification and comparison of novel genetic manipulation strategies for key Apicomplexan parasites.
  • Highlighting progress in overcoming limitations in genetic tractability.
  • Emphasis on advancements enabling functional genomics and high-throughput screening.

Conclusions:

  • Recent strategies have significantly advanced the genetic manipulation of Plasmodium and Toxoplasma species.
  • These advancements are crucial for understanding parasite biology and identifying novel therapeutic targets.
  • Further development of high-throughput methods will accelerate the discovery of gene function and drug targets.