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Updated: Jun 2, 2026
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
An amperometric biosensor based on ascorbate oxidase immobilized in poly(3,4-ethylenedioxythiophene)/multi-walled
Ming Liu1, Yangping Wen, Jingkun Xu
1Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, China.
Abstract:
An amperometric L-ascorbic acid (AA) biosensor fabricated by immobilizing ascorbate oxidase (AO) in poly(3,4-ethylenedioxythiophene) (PEDOT) and multi-walled carbon nanotubes (MWCNTs) composite films was reported for the first time. The entrapment of AO in PEDOT/MWCNTs composite films was performed during an electrochemical polymerization process. The influence of various experimental conditions was examined for determining the optimum analytical performance. The response of the biosensor towards AA under the optimized conditions is linear from 0.05 to 20 mM with a detection limit of 15 µM (S/N = 3). The biosensor shows a response time of 20 s and a sensitivity of 23.95 mA M(-1) cm(-2). The apparent Michaelis-Menten constant (K(m)) and apparent activation energy (E(a)) are 19.5 mM and 21 kJ mol(-1), respectively. Moreover, the biosensor exhibits good anti-interferent ability, good reproducibility and remarkable storage stability.
