Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Time-resolved EPR spectroscopy in a Unix environment.

N M Lacoff1, J E Franke, J T Warden

  • 1Department of Chemistry, Rensselaer Polytechnic Institute, Troy, New York 12180-3590.

Journal of Chemical Information and Computer Sciences
|February 1, 1990
PubMed
Summary

A new computer-aided time-resolved electron paramagnetic resonance (EPR) system was developed using Unix and C. This system enables efficient data acquisition and analysis, demonstrated with photosystem 1 research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variability of TSNA in U.S. Tobacco and Moist Smokeless Tobacco Products.

Toxicology reports·2020
Same author

Site of salicylaldoxime interaction with photosystem II.

Photosynthesis research·2014
Same author

Tetranitromethane modification of photosystem 2.

Photosynthesis research·2014
Same author

Characterization of publication rotogravure press emission rates and compositions.

Applied occupational and environmental hygiene·2001
Same author

Determination of VOC emission rates and compositions for offset printing.

Journal of the Air & Waste Management Association (1995)·1995
Same author

VOC emission rates and emission factors for a sheetfed offset printing shop.

American Industrial Hygiene Association journal·1995

Area of Science:

  • Biophysics
  • Spectroscopy
  • Computer Science

Background:

  • Time-resolved electron paramagnetic resonance (EPR) spectroscopy is crucial for studying fast biological processes.
  • Implementing advanced data acquisition systems in multi-user environments presents technical challenges.

Purpose of the Study:

  • To develop a computer-aided time-resolved EPR spectrometer.
  • To overcome challenges in real-time data acquisition within a Unix environment.
  • To demonstrate the system's utility in biological research.

Main Methods:

  • Interfacing a Varian E-9 EPR spectrometer and Biomation 805 waveform recorder with a PDP-11/23A minicomputer.
  • Utilizing the Unix operating system (version 2.9 BSD) and C programming language for system control and data analysis.

Related Experiment Videos

  • Addressing issues related to real-time data acquisition, memory management, and hardware control in a multitasking environment.

    • Main Results:

    • Successfully developed and implemented a functional computer-aided time-resolved EPR system.
    • Resolved technical challenges in real-time data acquisition and computer memory addressing under Unix.
    • Demonstrated system performance using time-resolved EPR spectra of photosystem 1 reaction center cation.

    Conclusions:

    • The developed Unix-based, C-programmed time-resolved EPR system offers an efficient solution for biological spectroscopy.
    • The system successfully overcomes common limitations in real-time data acquisition and hardware interfacing.
    • The approach provides a robust platform for advanced biophysical studies, as evidenced by its application to photosynthesis research.