Related Experiment Video
Updated: Jun 2, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
Published on: December 30, 2025
A new 42-kda protein-binding to the growth suppressor protein-p53
K Appel1, E Schneider, P Wagner
1UNIV SAARLAND,DEPT MED BIOCHEM,D-66421 HOMBURG,GERMANY. KAROLINSKA INST,DEPT CHEM 1,S-17177 STOCKHOLM,SWEDEN.
Abstract:
Expression of the growth suppressor gene p53 in Schizosaccharomyces pombe causes growth arrest which can be suppressed by overexpression of a yeast gene termed tms1. The tms1 gene encodes a 37 KDa protein which binds to p53 in vitro and in vivo when p53 was expressed in fission yeast cells. By using antibodies against recombinant tms1 protein we analyzed mammalian cells for the expression of a tms1 related protein. Human, monkey and rat cells exhibited a bright nuclear fluorescence when incubated with the tms1 specific antibody. In addition, we were able to specifically immunoprecipitate a 42 KDa protein which forms complexes with the p53 protein. The specificity of these complexes was demonstrated by coimmunoprecipitation using different monoclonal antibodies against p53, antibodies against yeast tms1 as well as by Western blot analysis. Thus, we found that the mammalian tms1 related protein represents a new p53 binding protein.
Related Concept Videos
Abnormal Proliferation
Negative Regulator Molecules
PI3K/mTOR/AKT Signaling Pathway
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein.
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Inhibition of Cdk Activity

