Related Experiment Video
Updated: Jun 2, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
Published on: December 30, 2025
Characterization of a mutated p53 exhibiting a cell cycle-related nuclear translocation
P Cahen1, D Grunwald, D Pigott
1CEA,DEPT BIOL MOLEC & STRUCT,INSERM,U309,BIOL MOLEC CYCLE CELLULAIRE LAB,F-38054 GRENOBLE 9,FRANCE.
Abstract:
We studied the p53 expression in the F4NW0 cell line. We found that this mutated p53 has a deletion of eight amino acids, between the conserved domains IV and V. Only one of the two alleles is expressed. It contains a single base mutation, which seems to promote the use of a cryptic splicing acceptor site, resulting in the observed deletion. The protein shows a cell cycle-dependent nuclear translocation: in G(1), it is exclusively cytoplasmic, and during the G(1)/S transition, it translocates into the nucleus. Nevertheless, different antibodies assayed revealed different patterns of localization.
Related Concept Videos
Abnormal Proliferation
DNA Damage Can Stall the Cell Cycle
DNA Damage can Stall the Cell Cycle
Negative Regulator Molecules
Positive Regulator Molecules
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

