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Updated: Jun 2, 2026

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Physical exercise at high altitude is associated with a testicular dysfunction leading to reduced sperm concentration
Fiore Pelliccione1, Vittore Verratti, Anatolia D'Angeli
1Andrology Unit, Department of Internal Medicine, University of L'Aquila, L'Aquila, Italy.
Objective:
To explore the effect of physical exercise at high altitudes (HA) on male reproductive system.
Design:
Prospective study.
Setting:
Andrology Clinic, University of L'Aquila, Italy.
Patient(S):
Seven male mountaineers involved in an expedition at 5,900 m.
Intervention(S):
Semen analysis, sperm DNA fragmentation with flow cytometry, and reproductive hormone levels.
Main Outcome Measure(S):
Hormone levels were evaluated at sea level (SL) at baseline (SL-pre), after 22 days of exercise at HA (intermediate), and after 10 days upon reaching SL (SL-post). Sperm parameters, percentage of sperm with fragmented DNA, and body composition measures were evaluated at SL-pre and at SL-post.
Result(S):
A reduction of sperm concentration, of body mass index (BMI), of waist circumference, and of percentage of body fat was observed at SL-post compared with SL-pre values. Increased levels of FSH and PRL were observed at the intermediate point, and normalized at SL-post, whereas T was higher at SL-post compared with SL-pre levels.
Conclusion(S):
Physical exercise at HA is associated with a testicular dysfunction leading to a reduced sperm concentration probably through an altered spermiation. The improved body composition after physical exercise might explain the higher T levels observed after the expedition.
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