Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Saturation Genome Editing reveals the functional impact of RAD51D <i>and</i> XRCC2 variants.

bioRxiv : the preprint server for biology·2026
Same author

Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis.

bioRxiv : the preprint server for biology·2026
Same author

Evidence for G6PD variant classification from multiplexed functional assays.

Genome biology·2026
Same author

Phenotype-Specific Recalibration of MAVE Data Enables Repurposing of <i>BAP1</i> Functional Assays for Küry-Isidor Syndrome.

medRxiv : the preprint server for health sciences·2026
Same author

Not Forgotten: Patient Experiences with Genetic Variant Reclassifications.

medRxiv : the preprint server for health sciences·2026
Same author

Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effects.

Cell·2026
Same journal

Make uphill thermodynamics downhill in pathway design.

Trends in biotechnology·2026
Same journal

Engineering a capture-bioremediate-release microbial biofilm for simultaneous bioremediation of microplastics and adsorbed heavy metals.

Trends in biotechnology·2026
Same journal

Engineered bacterial biofilms for biotechnological applications.

Trends in biotechnology·2026
Same journal

Multiscale and programmable engineering of edible mushroom mycelium-based materials.

Trends in biotechnology·2026
Same journal

Transporter engineering in microbial cell factories.

Trends in biotechnology·2026
Same journal

Random integration and high-throughput screening forging robust microbial cell factories.

Trends in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Deep mutational scanning: assessing protein function on a massive scale.

Carlos L Araya1, Douglas M Fowler

  • 1Department of Genome Sciences, 1705 NE Pacific St, University of Washington, Seattle, WA 98195, USA.

Trends in Biotechnology
|May 13, 2011
PubMed
Summary
This summary is machine-generated.

Deep mutational scanning uses high-throughput sequencing (HTS) to rapidly analyze thousands of protein mutants simultaneously. This powerful approach allows for comprehensive functional assessment, moving beyond traditional methods that only identify top-performing mutants.

More Related Videos

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Related Experiment Videos

Last Updated: Jun 2, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
11:36

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
08:46

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Area of Science:

  • Biochemistry and Molecular Biology
  • Protein Engineering
  • Genomics and Proteomics

Background:

  • Understanding protein function is crucial and often achieved through analyzing protein mutants.
  • Traditional protein display methods are limited, identifying only a few high-activity mutants.
  • This limits the scope of functional analysis and discovery of diverse mutant properties.

Purpose of the Study:

  • To review the emergence and impact of deep mutational scanning (DMS) in protein analysis.
  • To highlight the advantages of DMS over traditional protein display and selection methods.
  • To discuss the challenges and diverse applications of DMS in biological research.

Main Methods:

  • Leveraging protein display technologies for mutant generation and selection.
  • Integrating high-throughput sequencing (HTS) for simultaneous assessment of numerous mutants.
  • Deep mutational scanning enables analysis across the entire activity spectrum (high to low).

Main Results:

  • DMS allows for the simultaneous functional assessment of hundreds of thousands of protein mutants.
  • This approach provides a comprehensive view of the relationship between mutation and protein activity.
  • DMS is a rapid, cost-effective, and broadly applicable method for protein functional analysis.

Conclusions:

  • Deep mutational scanning represents a significant advancement in analyzing protein mutant libraries.
  • The integration of HTS with protein display offers unprecedented insights into protein function.
  • DMS has broad utility and exciting applications across various scientific domains.