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Published on: November 20, 2012
Early versus late GD-DTPA MRI enhancement in experimental glioblastomas
Paolo Farace1, Stefano Tambalo, Silvia Fiorini
1Department of Morphological-Biomedical Sciences, Section of Anatomy and Histology, University of Verona, Verona, Italy. paolofarace@anatomy.univr.it
Purpose:
To compare early versus late enhancement in two glioblastoma models characterized by different infiltrative/edematous patterns.
Materials And Methods:
Three weeks after inoculation into nude mice of U87MG and U251 cells, T1-weighted images were acquired early (10.5 min), intermediate (21 min) and late (30.5 min) after a bolus injection of Gd-DTPA at 300 μ mol/kg dosage. EARLY(TH) and LATE(TH) were the corresponding volumes with an enhancement higher than a threshold TH, defined by the mean (μ) and standard deviation (σ) on a contralateral healthy area. ADD(TH) was the enhancing volume found in LATE(TH) but not in EARLY(TH). T2 imaging of both tumors was performed, and T2 mapping of U251.
Results:
In all tumors, LATE(TH) was significantly higher than EARLY(TH) for TH ranging from μ+σ to μ+5σ. The ADD(TH) /EARLY(TH) ratio was not significantly different when U251 and U87MG tumors were compared. In the U87MG tumors, some enhancement was observed outside the regularly demarcated T2-hyperintense area. In the U251 tumors, irregularly T2 demarcated, a large portion of ADD(μ+3σ) had normal T2 values. At histology, U251 showed a higher infiltrative pattern than U87MG.
Conclusion:
In these models, the increase over time in the enhancing volume did not depend on the different infiltrative/edematous patterns and was not closely related with edema.
