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Published on: October 12, 2017
A highly sensitive RP-HPLC-fluorescence method to study aldehyde oxidase activity.
Mohammad-Reza Rashidi1, Kaveh Amini, Mohammad-Yaser Khani
1Tabriz University of Medical Sciences, School of Pharmacy, Department of Medicinal Chemistry, 51664-14766 Tabriz, Iran. rashidi@tbzmed.ac.ir
Journal of AOAC International
|May 14, 2011
Summary
A new RP-HPLC-fluorescence method accurately measures aldehyde oxidase (AO) activity in biological samples. This sensitive assay is crucial for understanding AO
Area of Science:
- Biochemistry
- Analytical Chemistry
- Pharmacology
Background:
- Aldehyde oxidase (AO) is a critical enzyme metabolizing diverse aldehydes and N-heterocycles.
- AO plays roles in physiological, pharmacological, and toxicological processes.
- Accurate measurement of AO activity is essential for biological and drug metabolism studies.
Purpose of the Study:
- To develop and validate a highly sensitive RP-HPLC-fluorescence method for assaying aldehyde oxidase activity.
- To establish a rapid and precise analytical technique for AO determination in biological matrices.
Main Methods:
- RP-HPLC with fluorescence detection was employed.
- Phenanthridine oxidation to phenanthridinone was used as the assay substrate.
- A C18 column with a specific mobile phase (phosphate buffer-acetonitrile) was utilized for separation.
Main Results:
- The method demonstrated high sensitivity, precision (RSD 2.54%), and accuracy.
- Linearity was observed from 0.5 to 100 nmol/L with a low limit of detection (LOD) of 0.125 nmol/L.
- Aldehyde oxidase activity was successfully measured in rat liver, kidney, ovary, and heart tissue fractions.
Conclusions:
- A validated, sensitive, and rapid RP-HPLC-fluorescence method for aldehyde oxidase assay has been established.
- This method is applicable for quantifying AO activity in various biological samples.
- The findings facilitate further research into AO's physiological and toxicological roles.
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