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Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
Intermolecular energy transfer from Tb3+ to Eu3+ in aqueous aggregates and on the surface of human cells
Minhee Lee1, Matthew S Tremblay, Steffen Jockusch
1Department of Chemistry, Columbia University, New York, New York 10027, United States.
Organic Letters
|May 14, 2011
Abstract:
Efficient intermolecular energy transfer from carbostyril 124-sensitized Tb(3+) to Eu(3+) in aqueous aggregates is reported. This energy transfer was also recapitulated on the cell surface of a human kidney cell line (HEK-293T) and imaged by fluorescence microscopy as an example for the applicability of this energy transfer probe for imaging in biological systems.
