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Updated: Jun 2, 2026

Extraction of Diatom DNA from Water Samples and Tissues
Published on: November 10, 2023
Ten microsatellite markers for the freshwater diatom Sellaphora capitata
Katharine M Evans1, Victor A Chepurnov, David G Mann
1Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh EH3 5LR, UK, SBAE Industries NV, Oostmoer 22A, 9950 Waarschoot, Belgium and Laboratory for Protistology and Aquatic Ecology, Ghent University, Krijgslaan 281-S8, 9000 Gent, Belgium.
Abstract:
Ten polymorphic microsatellite markers were developed for the benthic freshwater diatom Sellaphora capitata and tested on 40 isolates from a Belgian pond. Genotyping was very successful (95%). The number of alleles per locus ranged from three to 12 (mean 6.6) and expected heterozygosities from 0.2 to 0.86 (mean 0.67). This is the first time that microsatellite markers have been developed for a freshwater or benthic diatom.

