Microsatellite markers for the grapevine pathogen, Eutypa lata
K Baumgartner1, S E Bergemann, P Fujiyoshi
1USDA-ARS, 363 Hutchison Hall Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA Middle Tennessee State University, Biology Department, PO Box 60, Murfreesboro, TN 37132, USA, Department of Plant Science, University of Connecticut, Storrs, CT 06269, USA.
Abstract:
We isolated and characterized nine polymorphic microsatellite markers for Eutypa lata, a fungal pathogen responsible for Eutypa dieback of grapevine, in populations from two California vineyards (24 isolates per vineyard). Allele frequency ranged from two to 11 alleles per locus and haploid gene diversity ranged from 0.33 to 0.83. All samples comprised unique haplotypes. Our results suggest that there is sufficient allelic polymorphism to estimate fine-scale spatial structure, and to identify possible sources of inoculum from habitats outside of vineyards.
