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Microsatellite markers for the grapevine pathogen, Eutypa lata.

K Baumgartner¹, S E Bergemann, P Fujiyoshi

¹USDA-ARS, 363 Hutchison Hall Department of Plant Pathology, University of California, One Shields Avenue, Davis, CA 95616, USA Middle Tennessee State University, Biology Department, PO Box 60, Murfreesboro, TN 37132, USA, Department of Plant Science, University of Connecticut, Storrs, CT 06269, USA.

Molecular Ecology Resources

|May 14, 2011

Summary

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Researchers developed nine microsatellite markers for Eutypa lata, a grapevine pathogen. These markers reveal genetic diversity and can track the spread of Eutypa dieback disease in vineyards.

Area of Science:

Plant pathology
Molecular ecology
Mycology

Background:

Eutypa lata causes Eutypa dieback, a significant disease in grapevines.
Understanding the genetic structure of Eutypa lata populations is crucial for disease management.

Purpose of the Study:

To develop and characterize polymorphic microsatellite markers for Eutypa lata.
To assess the genetic diversity and population structure of Eutypa lata in California vineyards.

Main Methods:

Isolation and characterization of nine microsatellite loci from Eutypa lata.
Analysis of allele frequencies and haploid gene diversity in 48 isolates from two vineyards.
Haplotype analysis to determine genetic uniqueness.

Main Results:

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Nine polymorphic microsatellite markers were successfully developed for Eutypa lata.
High levels of genetic diversity were observed, with 2-11 alleles per locus and gene diversity ranging from 0.33 to 0.83.
Each sampled isolate possessed a unique haplotype, indicating high genetic resolution.

Conclusions:

The developed microsatellite markers are suitable for fine-scale spatial genetic analysis of Eutypa lata.
These markers can aid in identifying the origins of Eutypa dieback inoculum, including sources outside of vineyards.