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Development of nine polymorphic microsatellite markers for the phytoparasitic nematode Xiphinema index, the vector of
L Villate1, D Esmenjaud, S Coedel
1INRA, Agrocampus Rennes, Univ Rennes 1, UMR BiO3P 1099 (Biology of Organisms and Populations applied to Plant Protection), F-35653 Le Rheu, France, Equipe 'Interactions Plantes-Nématodes', UMR IBSV, INRA Sophia Antipolis, France, Ouest-Genopole Plateforme Genotypage - INRA UMR 118, Domaine de la Motte, BP 35327, F-35000 Rennes, France.
Abstract:
We report isolation, characterization and cross-species amplification of nine microsatellite loci from the phytoparasitic nematode Xiphinema index, the vector of grapevine fanleaf virus. Levels of polymorphism were evaluated in 62 individuals from two X. index populations. The number of alleles varies between two and 10 depending on locus and population. Observed heterozygosity on loci across both populations varied from 0.32 to 0.857 (mean 0.545). The primers were tested for cross-species amplification in three other species of phytoparasitic nematodes of the Xiphinema genus. These nine microsatellite loci constitute valuable markers for population genetics and phylogeographical studies of X. index.
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