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Updated: Jun 2, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Characterization of 32 novel microsatellite loci for population and mating system studies using Campostoma anomalum
J B Landis1, S P Hudman, M J Grose
1Department of Ecology and Evolutionary Biology Natural History Museum and Biodiversity Institute, Dyche Hall, University of Kansas, 1345 Jayhawk Boulevard, Lawrence, KS 66045, USA, Department of Biology, Truman State University, Kirksville, MO 63501, USA, 1637 Merion Place, Lawrence, KS 66047, USA.
Abstract:
The central stoneroller (Campostoma anomalum) is an abundant, widespread and sexually dimorphic stream minnow that is a useful model for mating system studies as well as a sentinel species for understanding population-level processes for fishes in headwater communities. We developed one genomic library enriched for dinucleotide repeats and isolated 48 putative, novel microsatellite loci. Of those, we present 32 polymorphic and independent microsatellite markers with 3 to 16 alleles per locus and heterozygosity ranging from 0.23 to 0.95. Hence, these markers will be useful for future behavioural, ecological and evolutionary studies using C. anomalum.
