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Updated: Jun 2, 2026
Laboratory Rearing of Stable Flies and Other Muscoid Diptera
Published on: August 3, 2018
Twenty-three new microsatellite loci in the stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae)
Marina S Ascunce1, C C Scotty Yang, Chris Geden
1US Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology (CMAVE), 1600 SW 23rd Drive, Gainesville, FL 32608, USA, Department of Entomology, National Taiwan University, No. 1, Sec 4, Roosevelt Road, Taipei 106, Taiwan.
Abstract:
The stable fly, Stomoxys calcitrans (L.), is a significant pest of cattle. Twenty-three microsatellite markers were isolated from a repeat-enriched genomic library of S. calcitrans. We characterized variation at these markers and found that 17 loci were polymorphic in two fly populations from Florida. Two to nine alleles were observed among the variable microsatellite loci and expected heterozygosities ranged from 0.03704 to 0.85115. These markers will be useful for characterizing population genetic differentiation and for tracking the migration patterns of stable flies in the USA and worldwide.
