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Updated: Jun 2, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

Polymorphic microsatellite markers in Euryale ferox Salisb. (Nymphaeaceae).

Zhiwu Quan¹, Lei Pan, Weidong Ke

¹Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China, Wuhan National Field Observation and Research Station for Aquatic Vegetables, Wuhan 430065, China.

Molecular Ecology Resources

|May 14, 2011

Summary

Researchers identified 11 microsatellite markers for Euryale ferox, an aquatic plant. These markers will aid in understanding the genetic diversity and population structure of this important species.

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Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

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Published on: October 5, 2017

Area of Science:

Plant genetics
Molecular biology
Ecology

Background:

Euryale ferox (Nymphaeaceae) is an aquatic plant belonging to basal Magnoliophyta.
Understanding the genetic diversity of E. ferox is crucial for its conservation and management.
Microsatellite markers are valuable tools for population genetic studies.

Purpose of the Study:

To isolate and characterize polymorphic microsatellite markers in Euryale ferox.
To assess the utility of these markers for population genetic analysis.

Main Methods:

Isolation and identification of 11 polymorphic microsatellite markers.
Genotyping of 34 individuals from a wild E. ferox population.
Analysis of allele number, expected heterozygosity, and observed heterozygosity.

Main Results:

Eleven polymorphic microsatellite markers were successfully identified in E. ferox.
A total of 25 alleles were detected across the 11 loci in the surveyed population.
Observed heterozygosity values were generally low, ranging from 0.000 to 0.088, while expected heterozygosity ranged from 0.056 to 0.634.

Conclusions:

The developed microsatellite markers are effective for studying the genetic structure of E. ferox populations.
These markers provide a foundation for future research into the population genetics and evolutionary history of E. ferox.