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Published on: December 5, 2016
Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria)
1Ecological Genetics Research Unit, Department of Biological and Environmental Sciences, University of Helsinki, PO Box 65, FI-00014 Helsinki, Finland.
Abstract:
We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandian populations and yielded an average of six to nine alleles per locus (range = 1-30) depending on the population. Average observed heterozygosities in the two populations were 0.16 (range = 0-0.91) and 0.36 (range = 0-1).
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