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Isolation and characterization of 11 microsatellite markers from Sagittaria latifolia (Alismataceae)
Sarah B Yakimowski1, Paul D Rymer, Harriet Stone
1Department of Ecology and Evolutionary Biology, University of Toronto, Toronto, Ontario, Canada M5S 3B2, Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, UK, Imperial College London, Silwood Park Campus, Ascot SL5 7PY, UK, Department of Biology, Trent University, Peterborough, Ontario, Canada K9J 7B8.
Abstract:
We developed 11 microsatellite loci for Sagittaria latifolia, an aquatic plant common to wetlands of North America. From an (AG)-enriched library, we identified 66 unique microsatellite sequences for which primers could be designed. Twenty-two loci reliably amplified a clear single band of expected size, and 11 loci were scoreable and polymorphic. For these 11 loci, we genotyped a monoecious and a dioecious population, yielding four to 14 alleles per locus. Three loci exhibited significant linkage disequilibrium leaving eight independent variable loci. Eight loci also amplified in four other Sagittaria species. These microsatellite loci will be useful to compare genetic structure among monoecious and dioecious populations of S. latifolia.
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