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Development of simple sequence repeat markers for Japanese isolates of Magnaporthe grisea
1National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi, Kumamoto 861-1192, Japan Life Science Research Center, Gifu University, Gifu 501-1193, Japan National Institute of Fruit Tree Science, Nagasaki 859-2501, Japan Faculty of Bioresources Sciences, Akita Prefectural University, Akita 010-0195, Japan.
Abstract:
We developed nine simple sequence repeat (SSR) markers useful for differentiating Japanese isolates of Magnaporthe grisea through a bioinformatic approach. Repeat sequences in the genome of M. grisea were identified by a Tandem Repeat Finding program. Length polymorphisms at 28 loci were examined, nine of which were selected on the basis of detected polymorphisms. These nine SSR markers showed a Nei's gene diversity ranging from 0.23 to 0.91 among 48 field isolates of two natural populations. These SSR markers are well suited for M. grisea epidemiology and population genetics.
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