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Novel microsatellite loci in the grass snake (Natrix natrix) and cross-amplification in the dice snake (Natrix
B Meister1, G F J Armbruster, N Frauenfelder
1Department of Environmental Sciences, Section of Conservation Biology (NLU), University of Basel, St. Johanns-Vorstadt 10, CH-4056 Basel, Switzerland Department of Environmental Sciences, Section of Plant Ecology, University of Basel, Schönbeinstrasse 6, CH-4056 Basel, Switzerland ecogenics GmbH, Wagistrasse 27, CH-8952 Schlieren, Switzerland.
Abstract:
Six novel polymorphic microsatellite loci are presented for the grass snake (Natrix natrix), a species with declining populations in many regions. The number of alleles per locus ranged from two to seven. Four dice snake (Natrix tessellata) microsatellites were polymorphic in the grass snake with three to four alleles. At two loci, the expected heterozygosity differed significantly from observed heterozygosity. Cross-amplification of the grass snake markers in the dice snake showed two polymorphic microsatellites with two and four alleles.
