Related Experiment Video
Updated: Jun 2, 2026
Avian Influenza Surveillance with FTA Cards: Field Methods, Biosafety, and Transportation Issues Solved
Published on: August 2, 2011
Generation of a Sequence Characterized Amplified Region Probe for Authentication of Mainland Serow (Capricornis
Kai Meng1, Jian Yu, Tao-Lin Luo
1College of Life Science, Anhui provincial key laboratory of the conservation and exploitation of biological resources and the Key Laboratory of Biotic Environment and Ecological safety in Anhui Province, Anhui Normal University, Wuhu 241000, China.
Abstract:
Mainland serow is an endanged artiodactyl of southern Anhui province, China, that is often subject to poaching. To provide an easy, rapid and reliable marker for identification of bushmeat, skin and other tissues of the species, we developed a sequence characterized amplified region (SCAR) based on a species-specific random amplified polymorphic DNA (RAPD) marker. Initially, a 1012-bp species-specific DNA fragment of mainland serow was detected by a RAPD primer S1193. Then, a serow-specific primer pair (SCF/SCR) was designed according to the specific RAPD fragment, resulting in a 438-bp SCAR for the species. Finally, the reliability of the SCAR primers was tested by a common multiplex polymerase chain reaction using the combination of the SCAR and cyt b universal primers. The results that all mainland serow samples presented two target bands but the others failed to produce the SCAR indicated that the designed primers were highly diagnostic. Therefore, the SCAR probe developed in this study will be useful for quick authentication of mainland serow tissue samples for conservation biology and bushmeat regulation.
