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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Microsatellite DNA markers for population-genetic studies of blue mackerel (Scomber australasicus) and cross-specific
C Y Tang1, C H Tzeng, C S Chen
1Research Centre for Biodiversity, Academia Sinica, Nankang, Taipei 115, Taiwan Institute of Zoology, Department of Life Science, National Taiwan University, Taipei 106, Taiwan Institute of Marine Affairs and Resource Management, National Taiwan Ocean University, Keelung 20224, Taiwan.
Abstract:
Blue mackerel (Scomber australasicus) is targeted by large-scale purse-seiners in the western North Pacific, and its stock structure is still contentious. Herein, we described 10 polymorphic microsatellite loci for blue mackerel. The number of alleles among 32 individuals surveyed ranged from five to 27 (average of 16.2 alleles per locus). Departures from Hardy-Weinberg expectation were observed at two loci. Cross-specific amplification in the congener, S. japonicus, was successful, except for one locus, revealed to be diagnostic for these congeners. These microsatellite loci will be useful tools to address queries in population genetic structure, fishery management unit and taxonomic species status in the genus Scomber.
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