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Sixteen polymorphic microsatellite markers from Zizania latifolia Turcz. (Poaceae)
Zhiwu Quan1, Lei Pan, Weidong Ke
1Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China Wuhan National Field Observation & Research Station for Aquatic Vegetables, Wuhan 430065, China.
Abstract:
Sixteen polymorphic microsatellite markers were isolated and identified in Zizania latifolia Turcz. (Poaceae), a perennial aquatic plant widespread in Eastern Asia. The microsatellite-enriched library was constructed using the fast isolation by AFLP of sequences containing repeats method. These markers revealed two to 14 alleles, with an average of 5.6 alleles per locus. The observed and expected heterozygosities varied from 0.071 to 0.690 and from 0.174 to 0.812, respectively. These markers will be useful for studying of gene flow and evaluating the genetic diversity of the Zizania latifolia population.
