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Microsatellites from the charcoal rot fungus (Macrophomina phaseolina)
Richard E Baird1, Phillip A Wadl, Xinwang Wang
1Entomology and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA, Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA, USDA-ARS Southern Horticultural Laboratory, Poplarville, MS 39470, USA, USDA-ARS-CGRU MSA, Stoneville, MS 38776, USA, University of Minnesota, Minneapolis, MN 54455 USA.
Abstract:
Microsatellite loci were identified from the charcoal rot fungus (Macrophomina phaseolina). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease-resistant soybean and other susceptible crops.
