New microsatellite loci for the endangered scalloped hammerhead shark, Sphyrna lewini
Holly A Nance1, Toby S Daly-Engel, Peter B Marko
1Department of Biological Sciences, 132 Long Hall, Clemson University, Clemson, South Carolina 29634, USA Hawaii Institute of Marine Biology, PO Box 1346, Kaneohe, Hawaii 96744, USA.
Abstract:
We isolated 15 microsatellite markers for the scalloped hammerhead shark, Sphyrna lewini. Loci were tested on 80 specimens of S. lewini from four Eastern Pacific samples. The number of alleles per locus ranged from 6 to 31 (mean = 14). Observed and expected levels of heterozygosity per locus ranged from 0.39 to 0.91 (mean = 0.70) and from 0.54 to 0.90 (mean = 0.76), respectively. No pairs of loci were in gametic disequilibrium after Bonferroni correction of α. One locus showed significantly lower heterozygosity than expected under Hardy-Weinberg proportions in two populations, possibly caused by null alleles.
