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Characterization of 27 microsatellite loci in the European flat oyster Ostrea edulis
Delphine Lallias1, Ruth Stockdale, Pierre Boudry
1School of Ocean Sciences, College of Natural Sciences, Bangor University, Menai Bridge, Anglesey LL59 5AB, UK, Ifremer, UMR M100 Physiologie et Ecophysiologie des Mollusques Marins, Plouzané, France, Ifremer, Laboratoire Génétique et Pathologie, Ronce-les-bains, 17390 La Tremblade, France.
Abstract:
The flat oyster Ostrea edulis is native to Europe and populations have been severely depleted by the parasite Bonamia ostreae since the 1980s. Additional genetic markers are required to improve population genetics study and linkage map development for selection for B. ostrea-resistance in this species. Here, we characterized 27 novel microsatellite loci for O. edulis. Number of alleles per locus ranged from 6 to 25 and observed heterozygosity between 0.375 and 1. Null alleles were suggested at a few loci but most loci were in Hardy-Weinberg agreement enabling their reliable use in further population and mapping genetics approaches.
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