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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Microsatellite loci within sixgill sharks, Hexanchus griseus
Shawn Larson1, Deborah Tinnemore, Chris Amemiya
1Seattle Aquarium, 1483 Alaskan Way, Pier 59, Seattle, WA 98101-2059, USA, Benaroya Research Institute at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101-2795, USA.
Molecular Ecology Resources
|May 14, 2011
Summary
Novel microsatellite loci were developed for sixgill sharks and tested across five shark species. Fourteen loci proved polymorphic and met Hardy-Weinberg equilibrium in sixgill sharks, aiding population genetics research.
Area of Science:
- Marine Biology
- Genetics
- Conservation Science
Background:
- Microsatellite markers are crucial for understanding elasmobranch population genetics.
- Previous microsatellite development has focused on species like the lemon shark, but novel markers are needed for other species, including the sixgill shark (Hexanchus griseus).
Purpose of the Study:
- To develop and characterize novel microsatellite loci for the sixgill shark.
- To assess the cross-species utility of these novel loci and previously developed lemon shark loci in five different shark species.
- To identify a robust set of polymorphic microsatellite markers for sixgill shark population studies.
Main Methods:
- Development of 15 novel microsatellite loci from sixgill shark genomic DNA.
- Testing of these loci, along with one lemon shark locus, on five species: sixgill shark, sevengill shark (Notorynchus cepedianus), Pacific spiny dogfish (Squalus acanthias), angle shark (Squatina californica), and leopard shark (Triakis semifasciata).
- Analysis of genetic diversity metrics including number of alleles (a), observed heterozygosity (H(O)), expected heterozygosity (H(E)), and F(IS) values for each species and locus.
Main Results:
- Data for 15 microsatellites are presented, detailing primer sequences and population genetic parameters for the tested species.
- A subset of 14 microsatellite loci (13 novel, 1 previously developed) were found to be polymorphic.
- These 14 microsatellite loci demonstrated conformity to Hardy-Weinberg equilibrium within the sixgill shark population.
Conclusions:
- The developed microsatellite loci are valuable genetic markers for the sixgill shark.
- The study provides a validated set of polymorphic microsatellites suitable for population genetic analyses of Hexanchus griseus.
- These markers can facilitate future research on the population structure, connectivity, and conservation of sixgill sharks and potentially other related species.
