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Updated: Jun 2, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Isolation and characterization of polymorphic microsatellite loci for the dace complex: Leuciscus leuciscus
Vincent Dubut1, Jean-François Martin, André Gilles
1Aix-Marseille Université, CNRS, IRD, UMR 6116 - IMEP, Equipe Persistance et Evolution de la Biodiversité, Centre Saint Charles, Case 36, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France. vincent.dubut@univ-provence.fr
Abstract:
Ten novel polymorphic microsatellites were isolated from the dace complex (Leuciscus leuciscus), which is a European cyprinid species. Four multiplex polymerase chain reaction sets were optimized in order to genotype 26 polymorphic loci in all, including 16 previously published cyprinid-specific loci. The level of genetic diversity was assessed in 142 dace individuals. We also successfully applied 26 of the microsatellites to 10 related species. These primers thus will be useful to assess population structure of the dace and other cyprinid species, with application for conservation issues and phylogeographical approaches.
